By John R. W. Masters
This new version of Animal phone tradition covers new or up to date chapters on telephone authentication, serum-free tradition, apoptosis assays, FISH, genetic amendment, scale-up, stem phone assays, three-dimensional tradition, tissue engineering and cytotoxicity assays. unique protocols for a large choice of equipment give you the middle of every bankruptcy, making new method simply available. each person operating in organic and scientific examine, even if in academia or a advertisement association, training mobile tradition will gain tremendously from this booklet.
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Extra resources for Animal Cell Culture: A Practical Approach
The second factor is whether this oxygen can be made available to the cells. The transfer rate of oxygen from the gas phase into a liquid phase has been calculated at about 17 (jug/cm2/h (8). Again, this is well in excess of that required by cells in a 1 litre flask. However, if the surface is assumed to be 26 SCALING-UP OF ANIMAL CELL CULTURES Table 1. 21 = proportion of oxygen in air; 32 = molecular weight of oxygen; 22400 = gram molecular volume; x 10-4 = solubility of oxygen in water at 37°C when equilibrated with air.
6 ug/ml) in under 1 h. It is necessary to supply oxygen to the medium throughout the life of the culture and the ability to do this adequately depends upon the oxygen transfer rate (OTR) of the system: where OTR is the amount of oxygen transferred per unit volume in unit time, Kl is the oxygen transfer coefficient, and a is the area of the interface across which oxygen transfer occurs (as this can only be measured in stationary and surfaceaerated cultures, the value JCla is used; this is the mass transfer coefficient (vol/h).
The only means of increasing the productivity of a roller bottle and decreasing its volume is by using a perfusion system, originally developed by Kruse et al. (15), and marketed by New Brunswick and Bellco (Autoharvester). This is an expensive option, as an intricate revolving connection has to be made for the supply lines to pass into the bottle. However, cell yields are considerably increased and extensive multilayering takes place. g. Costar, Bibby Sterilin) • Inverted microscope Method 1. Add 300 ml of growth medium.
Animal Cell Culture: A Practical Approach by John R. W. Masters